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KMID : 0545120150250091528
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 9 p.1528 ~ p.1531
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An Efficient PEG/CaCl2-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos
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Qiao Sun
Wei Wei
Juan Zhao
Jia Song
Fang Peng
Shaopeng Zhang
Yonglian Zheng
Ping Chen
Wenjun Zhu
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Abstract
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Sclerotia of Wolfiporia cocos are of medicinal and culinary value. The genes and molecular mechanisms involved in W. cocos sclerotial formation are poorly investigated because of the lack of a suitable and reproducible transformation system for W. cocos. In this study, a PEG/ CaCl2-mediated genetic transformation system for W. cocos was developed. The promoter Pgpd from Ganoderma lucidum effectively drove expression of the hygromycin B phosphotransferase gene in W. cocos, and approximately 30 transformants were obtained per 10 μg DNA when the protoplast suspension density was 106 protoplasts/ml. However, no transformants were obtained under the regulation of the PtrpC promoter from Aspergillus nidulans.
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KEYWORD
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Genetic transformation, medicinal and culinary value, promoter, Wolfiporia cocos
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